Cloning Vector

Stability and copy number in host bacteria of a new plasmid cloning vector

一种新的质粒克隆载体的稳定性和拷贝数的测定

Transformation - competent Artificial Chromosome ( TAC ) Cloning Vector and its Application Prospects in Fruit Genetic Engineering

可转化人工染色体（TAC）克隆载体及其在果树基因工程研究中的应用前景

Objective To clone and construct ANNEXIN A2 gene cloning vector .

目的克隆人类Annexina2基因，构建其正义真核表达质粒。

Conclusion : ① Rat antisense TIMP-1 gene TA cloning vector was constructed successfully .

结论：①成功地构建了含大鼠TIMP1反义基因片段的TA克隆载体；

Conclusion : The HLA-DR α gene TA cloning vector was contracted successfully .

结论成功地构建了HLA-DRα基因片段TA克隆载体。

Expression of antisense TIMP-1 gene TA cloning vector in rat glomerular mesangial cells

大鼠金属蛋白酶组织抑制物-1反义基因TA载体构建及其在肾小球系膜细胞表达的实验研究

First , cloning vector wais constructed : The gene encoding human insulin-like growth factor-I was modified .

首先，构建克隆载体：将改造的人胰岛素样生长因子（huIGF-I）基因直接克隆到T-载体，并进行DNA序列测定；

Construction of a neuregulin 1 cloning vector

构建Neuregulin1克隆载体的实验

Construction and Verification of the Effectiveness of pMBL : A Cloning Vector of Exported Proteins Encoding Genes

跨膜蛋白基因克隆载体pMBL的构建及验证

RESULTS : ① The cloning vector of target fragment was highly homologous with p21 gene promoter in cDNA sequence .

结果：①目的片段克隆载体与p21基因启动子cDNA序列高度同源。

An outline of the procedure for introducing a piece of foreign DNA into a cloning vector is shown in Figure 1.3 .

图1.3列出了引出一段外源DNA进入克隆载体的大概步骤。

Therefore , a new cloning vector ( T-vector ) was constructed , and specifically used to clone unmodified PCR products .

因此，我们构建了新的克隆载体即T载体，专门用于克隆未经任何修饰的PCR产物；

Objectives To construct the cloning vector of glycoprotein E1 gene of rubella virus , to study the mutation of glycoprotein E1 gene , and to analyze the sequence of E1 gene by the phylogenetic tree .

目的建立风疹病毒包膜糖蛋白E1的克隆载体；研究E1基因变异情况，并对其序列进行系统发生树分析。

Methods The genomic DNA of normal white blood cells and cultured K562 cells were respectively purified and digested with Sau3A ⅰ, and the digested DNA fragments of K562 cells were cloned into TA cloning vector to construct the corresponding genomic DNA library .

方法提取正常人白细胞和K562细胞基因组DNA，并用限制性内切酶Sau3AI酶切。

Cloning and Vector Construction of Human Insulin Gene

人胰岛素基因的克隆及其乳腺特异性表达载体的构建

By using the characteristics of anti-neomycin effect of the cloning expression vector , a minimal mortal dosage G418 was applied to obtain the neomycin resistant cell clone which showing human PDGF-B gene highly expressed .

利用重组质粒转染细胞的抗新霉素特性，经G418最小致死量筛选出抗性细胞克隆，免疫组织化学染色观察该细胞表达人PDGF-B基因情况。

Construction and identification of antisense HBV S gene cloning by T vector

反义乙型肝炎病毒S基因T载体克隆的构建

Hepatitis C Virus IRES Gene Cloning by T Vector and Sequencing

丙型肝炎病毒IRES基因T载体克隆及序列分析

Cloning and RNAi Vector Construction of Gene Fragments Related to Lignin Metabolism of Miscanthus

芒木质素代谢基因片段的克隆及RNAi载体构建

The cloning and recombinant vector constructing of lower-weight outer membrane protein encoding gene of Helicobacter pylori

幽门螺杆菌低分子质量外膜蛋白编码基因的克隆与重组载体构建

Gene cloning , prokaryotic vector constructing for the major allergen ovomucoid in Hen 's egg white and its recombinant expression

鸡蛋主要过敏原卵类粘蛋白基因的克隆及原核载体构建与表达

Cloning of expression vector for VEGF_ ( 121 ) and VEGF_ ( 165 ) gen es encoding human vascular endothelial growth factor

带信号肽人血管内皮生长因子基因VEGF（121）及VEGF（165）载体的克隆

Conclusion : The cloning and prokaryotic expression vector was successfully constructed .

结论：成功构建了血管基膜衍生多功能肽克隆和原核表达载体，并对其进行了空间结构分析。

Cloning and Sequencing and Vector Construction of the Main Structural Protein Gene of Goose Parvovirus

鹅细小病毒主要结构蛋白基因的克隆、测序及载体的构建

Gene cloning and prokaryotic expression vector construction of HLA-A mature peptide of extracellular domain gene

HLA-A胞外区成熟肽基因的克隆及其原核表达载体的构建

Cloning and Construction Expressing Vector of Ovine Interleukin-2 Gene

绵羊白细胞介素2基因的克隆与表达载体构建

Cloning and Construction Expressing Vector of D.nodosus serotype C Pili Gene

腐蹄病C型节瘤拟杆菌纤毛蛋白基因的克隆与表达载体的构建